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KMID : 0377220020270020081
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Medical Journal of Chosun Univercity
2002 Volume.27 No. 2 p.81 ~ p.88
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A Basic Study for the Development of a Multipurpose Culture Chamber
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Yang Nam-Woong
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Abstract
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Background and Objectives: An anaerobic chamber or jar for the anaerobic culture costs a great deal in upkeep, and most users are afraid of high-pressure gas tanks attached to a CO2 incubator or an anaerobic chamber. We devised the culture box with an air-bag and tested it to obtain the basic data for devising a multipurpose culture chamber which need not much upkeep expenses.
Materials and Methods: For tests, we manufactured polymethyl methacrylate box (280 × 280 × 240 mm, in size and 18.8 L in volume) with a polyvinyl chloride air-bag. The CO2 concentration inside the box was measured by a CO2 analyzer and an aspirating pump set. The O2 concentration was measured by an oxygen indicator inside the box and the dissolved oxygen concentration in the media by the dissolved oxygen meter.
Results: 5% CO2 was generated when 2.73 g of sodium bicarbonate and 6.83g of citric acid were mixed with 50 mL tap water inside the box. The absolute anaerobic condition was made within 45 minutes from mixing 3.5 g of sodium borohydride and 50 mL of 1 N acetic acid, and 5% O2 concentration (micro-aerobic condition) was produced within 15 minutes by mixing 20 mL of 1 N acetic acid and 1 g of sodium borohydride.
Conclusion: The culture box with an air-bag needs neither CO2 and mixed gas tanks, nor an expensive and disposable anaerobic pack. I think these results could be used for devising a new multipurpose culture chamber.
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KEYWORD
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Multipurpose culture chamber, Anaerobic culture, CO2 incubator
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